lea sistonen (Addgene inc)
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lea sistonen
Lea Sistonen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lea+sistonen/pcDNA-hHSF1+WT+(Plasmid+%2371724)/bio_rxiv__64898__2026__01__31__703055-187-7-9
Average 92 stars, based on 6 article reviews
Lea Sistonen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lea+sistonen/pcDNA-hHSF1+WT+(Plasmid+%2371724)/bio_rxiv__64898__2026__01__31__703055-187-7-9
Average 92 stars, based on 6 article reviews
lea sistonen - by Bioz Stars,
2026-09
92/100 stars
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Plasmid Preparation:Article Title: MNRR1 is required for Triple Negative Breast Cancer growth and metastasis and can be targeted by repurposed drugs Article Snippet: .. The pcDNA-hHSF1 WT was a gift from Article Title: The Transcriptional Repressor Orphan Nuclear Receptor TLX Is Responsive to Xanthines. Article Snippet: .. Plasmid Constructs. pFR-Luc was used as a reporter plasmid (Stratagene, San Diego, CA, U.S.A). pRL-SV40 (Promega, Fitchburg, WI, U.S.A.) was used for the normalization of cell growth and transfection efficiency and to observe test compound toxicity. pECE-SV40-Gal4-VP16 was a gift from Article Title: Screening of Chelidonium majus isoquinoline alkaloids reveals berberine and chelidonine as selective ligands for the nuclear receptors RORβ and HNF4α, respectively. Article Snippet: .. For the hybrid reporter gene assays, the previously reported Gal4‐fusion receptor plasmids pFA‐CMV‐THRα‐LBD,[46] pFA‐CMV‐THRβ‐ LBD,[46] pFA‐CMV‐hRARα‐LBD,[47] pFA‐CMV‐hRARβ‐LBD,[47] pFA‐ CMV‐hRARγ‐LBD,[47] pFA‐CMV‐hPPARα‐LBD,[48] pFA‐CMV‐hPPARδ‐ LBD,[48] pFA‐CMV‐hPPARγ‐LBD,[48] pFA‐CMV‐hRORα‐LBD,[49] pFA‐ CMV‐hRORβ‐LBD,[49] pFA‐CMV‐hRORγ‐LBD,[49] pFA‐CMV‐hVDR‐ LBD,[47] pFA‐CMV‐hPXR‐LBD,[47] pFA‐CMV‐hCAR‐LBD,[47] pFA‐CMV‐ hLXRα‐LBD,[50] pFA‐CMV‐hLXRβ‐LBD,[50] pFA‐CMV‐hFXR‐LBD,[51] pFA‐CMV‐hHNF4α‐LBD,[23] pFA‐CMV‐hRXRα‐LBD,[47] pFA‐CMV‐ hRXRβ‐LBD,[47] pFA‐CMV‐hRXRγ‐LBD,[47] pFA‐CMV‐hTLX‐LBD,[52] pFA‐CMV‐hNur77‐LBD,[53] pFA‐CMV‐hNurr1‐LBD,[53] pFA‐CMV‐ hNOR1‐LBD,[53] pFA‐CMV‐hLRH1‐LBD,[54] and pFA‐CMV‐hSF1‐LBD coding for the hinge region and LBD of the canonical isoform of the respective nuclear receptor were used. pFR‐Luc (Stratagene) was used as reporter plasmid and pRL‐SV40 (Promega) was used in all assays for normalization of transfection efficiency and cell growth. pECE‐SV40‐ Gal4‐VP16 was a gift from Article Title: Development of a Potent Nurr1 Agonist Tool for In Vivo Applications. Article Snippet: This project was financially supported by Immunic AG, Gräfelfing, Germany. .. Gal4-VP16 was a gift from Article Title: Comparative Profiling and Chemogenomics Application of Chemical Tools for NR4A Nuclear Receptors. Article Snippet: The ligand-activated transcription factors of the NR4A family are implicated as promising drug targets with neuroprotective and anticancer potential and attract strong attention in drug discovery.. Several NR4A modulators have been described, but a validated set of direct ligands for biological studies is lacking.. Here, we profiled the reported and commercially available agonists and inverse agonists under uniform conditions in several orthogonal test systems to establish a highly annotated tool and gain comprehensive insights into the NR4A modulator characteristics. Article Title: Nurr1 Modulation Mediates Neuroprotective Effects of Statins. Article Snippet: .. Gal4VP16 (Addgene plasmid # 71728) was a gift from Article Title: Scaffold Fusion and SAR Transfer with a Chemical Language Model Generates Novel Liver X Receptor Modulators Article Snippet: HEK293T and HepG2 cells (German Collection of Microorganisms and Cell Cultures, DSMZ) were grown in Dulbecco’s modified Eagle’s medium (DMEM) high glucose supplemented with 10% fetal bovine serum (FBS), sodium pyruvate (1 mM), penicillin (100 U/mL), and streptomycin (100 μg/mL) at 37 °C and 5% CO 2 . .. For the hybrid reporter gene assays, the previously reported Gal4-fusion receptor plasmids pFA-CMV-hRORα-LBD, pFA-CMV-hRORβ-LBD, pFA-CMV-hRORγ-LBD, pFA-CMV-hLXRα-LBD, pFA-CMV-hLXRβ-LBD, pFA-CMV-hFXR-LBD, pFA-CMV-hPXR-LBD, and pFA-CMV-hRXRα-LBD, coding for the hinge region, and LBD of the canonical isoform of the respective nuclear receptor were used. pFR-Luc (Stratagene) was used as the reporter plasmid, and pRL-SV40 (Promega) was used in all assays for the normalization of transfection efficiency and cell growth. pECE-SV40-Gal4-VP16 was a gift from Article Title: The Transcriptional Repressor Orphan Nuclear Receptor TLX Is Responsive to Xanthines. Article Snippet: A.C., X.N., and S.K. are grateful for support by the SGC, a registered charity (no: 1097737) that receives funds from AbbVie, Bayer AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genentech, Genome Canada through Ontario Genomics Institute [OGI-196], EU/EFPIA/ OICR/McGill/KTH/Diamond, Innovative Medicines Initia- tive 2 Joint Undertaking [EUbOPEN grant 875510], Janssen, Merck KGaA, Pfizer, Saõ Paulo Research Foundation-FAPESP, and Takeda. .. Gal4-VP16 was a gift from Construct:Article Title: The Transcriptional Repressor Orphan Nuclear Receptor TLX Is Responsive to Xanthines. Article Snippet: .. Plasmid Constructs. pFR-Luc was used as a reporter plasmid (Stratagene, San Diego, CA, U.S.A). pRL-SV40 (Promega, Fitchburg, WI, U.S.A.) was used for the normalization of cell growth and transfection efficiency and to observe test compound toxicity. pECE-SV40-Gal4-VP16 was a gift from Transfection:Article Title: The Transcriptional Repressor Orphan Nuclear Receptor TLX Is Responsive to Xanthines. Article Snippet: .. Plasmid Constructs. pFR-Luc was used as a reporter plasmid (Stratagene, San Diego, CA, U.S.A). pRL-SV40 (Promega, Fitchburg, WI, U.S.A.) was used for the normalization of cell growth and transfection efficiency and to observe test compound toxicity. pECE-SV40-Gal4-VP16 was a gift from Article Title: Screening of Chelidonium majus isoquinoline alkaloids reveals berberine and chelidonine as selective ligands for the nuclear receptors RORβ and HNF4α, respectively. Article Snippet: .. For the hybrid reporter gene assays, the previously reported Gal4‐fusion receptor plasmids pFA‐CMV‐THRα‐LBD,[46] pFA‐CMV‐THRβ‐ LBD,[46] pFA‐CMV‐hRARα‐LBD,[47] pFA‐CMV‐hRARβ‐LBD,[47] pFA‐ CMV‐hRARγ‐LBD,[47] pFA‐CMV‐hPPARα‐LBD,[48] pFA‐CMV‐hPPARδ‐ LBD,[48] pFA‐CMV‐hPPARγ‐LBD,[48] pFA‐CMV‐hRORα‐LBD,[49] pFA‐ CMV‐hRORβ‐LBD,[49] pFA‐CMV‐hRORγ‐LBD,[49] pFA‐CMV‐hVDR‐ LBD,[47] pFA‐CMV‐hPXR‐LBD,[47] pFA‐CMV‐hCAR‐LBD,[47] pFA‐CMV‐ hLXRα‐LBD,[50] pFA‐CMV‐hLXRβ‐LBD,[50] pFA‐CMV‐hFXR‐LBD,[51] pFA‐CMV‐hHNF4α‐LBD,[23] pFA‐CMV‐hRXRα‐LBD,[47] pFA‐CMV‐ hRXRβ‐LBD,[47] pFA‐CMV‐hRXRγ‐LBD,[47] pFA‐CMV‐hTLX‐LBD,[52] pFA‐CMV‐hNur77‐LBD,[53] pFA‐CMV‐hNurr1‐LBD,[53] pFA‐CMV‐ hNOR1‐LBD,[53] pFA‐CMV‐hLRH1‐LBD,[54] and pFA‐CMV‐hSF1‐LBD coding for the hinge region and LBD of the canonical isoform of the respective nuclear receptor were used. pFR‐Luc (Stratagene) was used as reporter plasmid and pRL‐SV40 (Promega) was used in all assays for normalization of transfection efficiency and cell growth. pECE‐SV40‐ Gal4‐VP16 was a gift from Article Title: Scaffold Fusion and SAR Transfer with a Chemical Language Model Generates Novel Liver X Receptor Modulators Article Snippet: HEK293T and HepG2 cells (German Collection of Microorganisms and Cell Cultures, DSMZ) were grown in Dulbecco’s modified Eagle’s medium (DMEM) high glucose supplemented with 10% fetal bovine serum (FBS), sodium pyruvate (1 mM), penicillin (100 U/mL), and streptomycin (100 μg/mL) at 37 °C and 5% CO 2 . .. For the hybrid reporter gene assays, the previously reported Gal4-fusion receptor plasmids pFA-CMV-hRORα-LBD, pFA-CMV-hRORβ-LBD, pFA-CMV-hRORγ-LBD, pFA-CMV-hLXRα-LBD, pFA-CMV-hLXRβ-LBD, pFA-CMV-hFXR-LBD, pFA-CMV-hPXR-LBD, and pFA-CMV-hRXRα-LBD, coding for the hinge region, and LBD of the canonical isoform of the respective nuclear receptor were used. pFR-Luc (Stratagene) was used as the reporter plasmid, and pRL-SV40 (Promega) was used in all assays for the normalization of transfection efficiency and cell growth. pECE-SV40-Gal4-VP16 was a gift from Gene Expression:Article Title: The Transcriptional Repressor Orphan Nuclear Receptor TLX Is Responsive to Xanthines. Article Snippet: .. Plasmid Constructs. pFR-Luc was used as a reporter plasmid (Stratagene, San Diego, CA, U.S.A). pRL-SV40 (Promega, Fitchburg, WI, U.S.A.) was used for the normalization of cell growth and transfection efficiency and to observe test compound toxicity. pECE-SV40-Gal4-VP16 was a gift from |